Graphene oxide nanofilm and chicken embryo extract decrease the invasiveness of HepG2 liver cancer cells

Abstract Background The extracellular matrix (ECM) is a mosaic of various structural and functional proteins that cooperate with the cell, regulate adhesion, consequently manage its further fate. Liver destruction accompanied by disruption physicochemical properties ECM which deregulates cell–ECM interaction can lead to uncontrolled proliferation neoplastic transformation cells. Therefore, it be assumed modification restoration characteristics for healthy tissue may counteract cell proliferation. purpose presented research model was optimise physical introducing graphene oxide plane/nanofilm (nfGO) enriching environment potentially missing adding protein cocktail (chicken embryo liver extract) determine impact these factors on cooperation consequences proliferation, phase, are invasiveness cancer Results Experiments were performed non-cancer HS-5 cells HepG2 C3A. divided into four groups: (1) control, (2) cultured nfGO, (3) addition chicken extract (CELE) (4) nfGO CELE. CELE contained 1735 proteins; top 57 have been presented. use as well + reduced greatest extent; this in contrast also C3A Furthermore, combined GO substrate effectively resulted decrease population G0/G1 phase an increase G2/M. Molecular analysis showed expression genes responsible adhesion such focal kinase ( fak ), e-cadherin, n-cadherin β-catenin , considered proto-oncogene. Conclusions Studies shown both surface structure grown presence multi-component natural regulatory proteins, modify integrins, and, consequence, cycle—entering resting phase. For first time, has documented hepatic line under influence stimuli derived from mimic (graphene oxide) unique complex decreased